Effect of continued exposure to drugs on gene expression in human pancreatic cells

Pancreatic ductal adenocarcinoma (PDAC) has a bad prognosis, a fast progression and is difficult to treat, highlighting the need for new therapeutic targets. Aggressive tumors mostly show Epithelial-to-Mesenchymal Transition (EMT). In a previous study we could show that Protein phosphatase type 2A (PP2A) plays a central role in processes of cell survival and proliferation and a link with EMT and tumor progression. In a separate study, we have investigated the effect of short and continuous inhibition of PP2A with Okadaic Acid on gene and protein expression in the human pancreatic cell line PANC-1. In this study, we investigated the cell line models of Panc-1 cells made resistant to the PP2A-small activator proteins ATUX and SMAP2. We performed gene expression profiling analysis using data obtained from RNA-seq of 3 different cells and compared it to control 4 different Panc-1 controls. Overall design: Comparative gene expression profiling analysis of RNA-seq data for PP2A-activators SMAP, ATUX and Sorafenib derived cell lines