Gene expression profile of embryonic stem cell-derived TS-like cells by RNA-sequencing

Human embryonic stem cells (ESCs) were differentiated into GATA3-expressing extraembryonic cells (GATA3+ ExE cells) by exposure to chemical inhibitors targeting ACTIVIN/NODAL (A83-01, 1uM) and FGF signaling (PD03714, 100nM). Using these GATA3-expressing cells as a starting material, we established trophoblast stem-like cells (TSLCs) following the established protocol for deriving trophoblast stem cells (TSCs) from the villous trophoblast of human first-trimester placenta. To characterize the resulting ESC-derived TSLCs, RNA sequencing was conducted, allowing comparisons with the parental ESCs and previously published TSCs. Overall design: We profiled mRNA expression in ESC-derived TSLCs at two different passage numbers, each with two biological replicates, to comprehensively assess their cellular state.