Structural insights into pre-pore intermediates of alpha-hemolysin in the lipidic environment

Cryo-EM map and atomic model derived from 1. Cryo-EM map of Alpha-hemolysin heptameric pore state derived from 10:0 PC liposomes. 2. Cryo-EM map of Alpha-hemolysin heptameric pre-pore state derived from 10:0 PC liposomes. 3. Cryo-EM map of Alpha-hemolysin heptameric late pre-pore state derived from 10:0 PC/Sphingomyelin liposomes. 4. Cryo-EM Density map of Staphylococcus aureus alpha-hemolysin pore structure derived from 12:0 Phosphatidylcholine (12:0 PC) liposome. 5. Cryo-EM Density map of Staphylococcus aureus alpha-hemolysin 12:0 PC liposome derived pre-pore structure of alpha hemolysin. 6. Alpha-hemolysin heptameric pore state derived from egg PC/Cholesterol (3:1 molar ratio) liposomes. 7. Structure of Alpha-hemolysin heptameric pore state derived from Egg-PC/SM liposomes 8. Map of EggPC/SM derived prepore structure of alpha-hemolysin