RNASeq analysis for T-cell blasts from individuals with an AIOLOS Q402* mutation and healthy controls

RNA-seq was performed using RNA extracted from enriched T-cell blasts for the following study groups: healthy controls (NC, n=5) and patients with AIOLOS Q402* (n=3 ). Illumina libraries were generated using the SMARTer Stranded Total RNA-Seq Kit v3 – Pico Input Mammalian Components (Takara San Jose, CA). Libraries were sequenced on a Illumina HiSeq 2500 instrument using 2 x 75 bp paired-end reads, and alignment and mapping analyses were performed by the Takara Cogent NGS Analysis Pipeline v1.5.0 (also known as CogentAP). Differential gene expression analysis was performed by DESeq2 v1.32.0 [Bioconductor version: Release (3.15)]. Overall design: Comparative gene expression profiling analysis of RNA-seq data for T-cell blasts from individuals with an AIOLOS Q402* mutation and healthy controls