TAZ ChIP-sequencing in GBM cells

ChIP-Sequencing for the transcription factor TAZ (WWTR1) was performed to define genome-wide TAZ binding sites in GBM stem-like cells. Cells after EGF treatment for 4 hours were subjected to TAZ ChIP using the MAGnify ChIP system (Invitrogen) following the manufacturer’s protocol. TAZ-associated DNA was immunoprecipitated using rabbit anti-TAZ antibody (Cell Signaling) and Dynabeads magnetic beads (Invitrogen). ChIP-enriched DNA or input DNA (10 ng each) was subjected to library preparation using a ChIP-Seq DNA Sample Prep kit (Illumina). Sequencing was performed using an Illumina HiSeq 2500 platform.