Hordeum vulgare cultivar:Hopum Genome sequencing

This project aims to identify quantitative trait loci (QTLs) associated with powdery mildew resistance in barley and to develop CAPS markers for marker-assisted selection. Using Bulked Segregant Analysis coupled with whole-genome resequencing, a major resistance QTL was identified and a candidate gene was predicted based on reference genome alignment (Morex) and gene expression analysis. The developed CAPS marker was further validated in resistant and susceptible barley lines. This study provides valuable molecular tools and genetic resources for improving disease resistance in barley breeding programs.