Brain RNA-seq: Esomeprazole/stress interactions.. Brain RNA-seq: Esomeprazole/stress interactions.

Purpose: The goals of this study were to evaluate the effects of acid suppression on the brain transcriptomer in a murine model of stress. In addition, the effects of the proton pump inhibitor (PPI), esomeprazole, on the brain transcriptome were evaluted in the presence or absence of stress. Methods: Male C57BL/6 mice were subjected to three days of stress by hypothermic immobilization or control environment for three hours daily and either esomeprazole 2 mg/kg or saline by intraperitoneal injection daily. Standard RNA-seq methods were employed to assess mRNA expression in the hippocampus and frontal cortex. Results: There was no effect of stress or esomeprazole on the frontal cortex transcriptome. Stress had no impact on the hippocampus transcriptome, but the addition of esomeprazole induced differential expression of 124 genes, many of which are involved in cognitive and behavior pathways. Conclusions: Further studies are needed to evaluate the associations between microbiota, host gene expression, the abundance of CNS neurocognitive modulators, and their impact on cognition and behavior. Overall design: mRNA from hippocampus and frontal cortex was profiled using Illumina HiSeq 2000 (2x151). Tissue was generated from 4 groups of 6 male C57BL/6 mice: 1) control environment plus vehicle; 2) control environment plus esomeprazole (2 mg/kg); 3) stress environment plus vehicle; 4) stress environment plus esomeprazole (2 mg/kg).