Using RNAseq for identifying hepatic genes involved in multi- (F1 generation) and transgenerational (F2 generation) responses to chronic benzo[a]pyrene exposure (F0 generation) in Xenopus tropicalis

We investigated the multi- and transgenerational impact of BaP on Xenopus females following F0 generation exposure from the tadpole to the mature adult stage at a concentration (50 ng.L-1). 5 females from F1 and F2 generations by parentale xposure conditions (control vs BaP) were used for studying liver transcriptome by RNAseq. RNA-seq libraries were sequenced on an illumina GAIIx sequencer as 75bp single reads mRNAseq from liver of mature F1 and F2 females.