Effect of Cardiac LIM Protein (CSRP3) on human proximal tubule epithelial cells

Cardiac LIM protein (CSRP3) is released from the heart after cardiac injury and transits the plasma to the renal filtrate. CSRP3 has a well described transcriptionally-active role in myocardiocyte development and response to stress. Although it appears in endocytic vesicles within tubular epithelial cells in vivo it is unknown whether it alters tubular epithelial cell transcription. Therefore to test the hypothesis that CSRP3 alters PTEC transcription, human primary PTEC were exposed to physiologic concentration of CSRP3 (10nM) for 6h prior to bulk RNA sequencing. For bulk RNA sequencing, hPTECs were treated with 10 nM rCSRP3 for 6h following 24h serum starvation. After rCSRP3 treatment, cells were harvested, then total RNA was extracted. hPTEC were treated with either PBS or CSRP3 and then subjected to RNA isolation and bulk RNA sequencing.