TABLE 1 in BlasTax-a user-friendly stand-alone tool to leverage the BLAST + program for molecular taxonomy

TABLE 1. Examples of open-source programs implementing the BLAST algorithm. Program / WrapperBLAST+ FunctionsOSReferenceBlastGuiGUI-based building of BLAST databases, sequence filtering and alignment.Windows, macOS, LinuxDu et al. (2020)Blast2GOBLAST for functional annotation. GO mapping & annotation. Visualization tools.Windows, macOS, Linux (Java-based)Conesa et al. (2005)BRIGBLAST Ring Image Generator. Compares prokaryote genomes based on BLAST results.Windows, macOS, LinuxAlikhan et al. (2011)NBLASTGUI-based tool implementing a two-way system that allows using of input sequences either as “query” or “target”. Includes dot plot analysis.WindowsMohanty et al. (2022)omicRBLASTn alignment of sequences against a reference. Results added to the original tabular format.WindowsTalamantes-Becerra et al. (2021)prfectBLASTGUI-based access to all basic BLAST+ functions.Windows, macOS, Linux (Java based)Santiago-Sotelo & Ramirez-Prado (2021)TBtoolsToolkit with many functions for big-data molecular analyses, including BLAST and interactive data visualizationWindows, macOS, Linux (Java based)Chen et al. (2020)UGENERun blastn, blastp, blastx etc. locally. Graphical BLAST viewer. Database management. Workflow integration.Windows, macOS, LinuxOkonechnikov et al. (2012)GalaxyBLAST+ wrappers for all tools. Multi-sequence queries. Pipeline integration. Visualization via Galaxy plugins.Web-based (server-hosted), installable on LinuxCock et al. (2015)PLAN (Personal BLAST Navigator)Query and target sequence database management. Automated high-throughput BLAST searching. Indexing, searching, filtering annotating results.Web-based applicationHe et al. (2007)BLASTGrabberGUI viewer for local BLAST+ results. Hit filtering, sorting, and visualization. Batch parsing (does not directly implement the BLAST algorithm itself).Windows, LinuxNeumann et al. (2014)blastjsBLAST+ wrapper for Node.js. BLAST+ functionality for tools based on JavaScript and Node.js.Node.js libraryPage et al. (2016)W.ND-BLASTDistributes BLAST queries to various nodes/ computers across local area networks (LAN). Intuitive GUIs for database creation, execution, output evaluation and result exportation.WindowsDowd et al. (2005)