RNAseq on uhrf1 zebrafish mutant larvae at 5 dpf compared to phenotypically normal siblings

In this study, we performed RNA-seq on 5 dpf whole larvae of uhrf1-hi272 mutants and phenotypically wild-type siblings to determine changes in the transcriptomic profile. To perform RNA-seq on pools of whole larvae of uhrf1-hi272 mutants and siblings on 5 dpf, we collected between 10 and 20 larvae from 2 independent clutches of uhrf1-hi272 mutants their phenotypically WT siblings which were sorted based on phenotype following immobilization using triacane. We extracted total RNA from the entire larvae to generate libraries, which were analyzed on an Agilent 2100 Bioanalyzer. Illumina TruSeq RNA sample preparation version 2 protocol with Ribo-Zero Gold. cDNA libraries were sequenced on the Illumina NextSeq500 platform to obtain 75 bp single-end reads.