Genomic changes in C. jejuni NCTC 11168-GSv in vitro and in vivo (relative to the sequence data for NCTC 11168-GS in GenBank [10]).

aAlteration location where the first letter indicates the original amino acid, the second letter indicates the substituted amino acid, and the number indicates the position at which the amino acid substitution occurred.bFrequency data denotes potential variants as determined through Multiple-Sequence alignment. +/− data denotes validated variants determined through a Mapping-Assembly using MIRA.cCampylobacter jejuni genomes: (A) NCTC 11168-GSv; (B) NCTC 11168-GSv after four transfers in vitro; (C) Kf1 [collective isolates-human host passage]; (D) Kf2 [collective isolates-human host passage]; (E) K12E5 [single isolate-human host passage]; (F) mcK12E5 [collective isolates from the ceca of mice after three passages]; and (G) mfK12E5 [collective isolates from feces of mice after three passages].dDenotes a hypervariable region within the genome of NCTC 11168-GS.eLocus unknown.fNo protein information available.gStop codon.h≥15% difference in A versus B.i≥15% difference in C or D versus A (i.e. genetic variation due to human passage).j≥15% difference in E versus the mean of C and D (i.e. genetic variation due to human passage).k≥15% difference in F or G versus E consistent with C and D (i.e. genetic variation due to murine passage).