Evaluation of transmission-blocking activity of purified mouse IgG.

(a)Protein G-purified polyclonal IgG from mice were used at a final concentration of 1 mg/ml. Polyclonal IgG purified from mice immunised with a non-malaria-related antigen was used as a negative control (Control). The antisera used originated from mice either immunised with the RFP-Pf38 fusionprotein or with Pf38 alone.(b)A Mann-Whitney U test was used to analyse the median numbers of oocysts between groups of mosquitoes either receiving control IgG or test IgG during infection with P. falciparum. P-values below 0.05 were considered significant.(c)Fisher's exact test was used to analyse the oocyst prevalence between groups of mosquitoes either receiving control IgG or test IgG during infection with P. falciparum.P-values below 0.05 were considered significant.