Comparison of RNA sequencing and qRT-PCR of ROBO1 and SLIT2 genes affected by knockdown.

Gene expression changes after SLIT2/ROBO1 knockdown were verified in a larger number of specimens (n = 6) by qRT-PCR. SLIT2 and ROBO1 were silenced in HTR8/SVneo commercial cell line with siRNAs. mRNA levels of SLIT2/ROBO1-silenced cells were compared with mRNA levels of cells treated with negative siRNA. In addition to genes affected by gene knockdown according to RNA sequencing data, we tested the effect of knockdown on mRNA expression of well-known cytokines IL-6 and TNF-A.