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Peripheral nerve injury may result in muscle atrophy and impaired motor function recovery, and numerous pieces of evidence indicate that long noncoding RNAs (lncRNAs) play crucial roles in skeletal muscle regeneration. Our preliminary sequencing results showed that LNC_000280 was significantly down-regulated in denervated mouse skeletal muscle and we hypothesized that LNC_000280 may play an important role in skeletal muscle regeneration. In this research, flow cytometry and EdU staining results showed that overexpression of LNC_000280 promoted the proliferation of C2C12, while knockdown LNC_000280 had the opposite effect. Knockdown LNC_000280 inhibited the differentiation of C2C12 cells. LNC_000280 regulated the expression of proliferation genes (Cdk2, Cdc27) and differentiation genes (MyoG, MyoD). GO analysis and PPI network of LNC_000280 target genes showed that LNC_000280 mainly regulates skeletal muscle cell metabolism, mitochondrial and muscle growth. Idh2, Klhl31, Agt, and Gpt2 may be important downstream targets for its function. Therefore, we believe that that LNC_000280 can regulate the proliferation and differentiation of myoblasts by regulating gene expression.

周围神经损伤可能导致肌肉萎缩及运动功能恢复障碍，众多证据表明长非编码RNA（lncRNA）在骨骼肌再生过程中扮演着至关重要的角色。本研究初步测序结果显示，LNC_000280在去神经化的小鼠骨骼肌中显著下调，我们推测LNC_000280可能在骨骼肌再生中发挥重要作用。本研究中，流式细胞术和EdU染色结果显示，LNC_000280的过表达促进了C2C12细胞的增殖，而LNC_000280的敲低则产生了相反的效果。敲低LNC_000280抑制了C2C12细胞的分化。LNC_000280调控了增殖基因（Cdk2、Cdc27）和分化基因（MyoG、MyoD）的表达。GO分析和LNC_000280靶基因的PPI网络分析表明，LNC_000280主要调控骨骼肌细胞的代谢、线粒体和肌肉生长。Idh2、Klhl31、Agt和Gpt2可能是其功能的重要下游靶点。因此，我们认为LNC_000280可以通过调控基因表达来调节肌原细胞的增殖和分化。