Genome-wide analysis of H3K4me3 in NOS1+ mouse enteric neurons

Neuronal nitric oxide synthase 1 (NOS1) produces the gaseous signaling molecule nitric oxide (NO), which plays important roles in the development and function of the nervous system. The regulation of Nos1 gene expression is incompletely understood. Here, we analyzed the genome-wide distribution of the histone mark H3K4me3 in FACS-purified nitrergic enteric neurons by chromatin immunoprecipitation-sequencing. Libraries were made from chromatin-immunoprecipitated DNA isolated from sorted NOS1+ mouse enteric neurons using anti-H3K4me3 antibodies and sequenced on Illumina HiSeq 4000 platform.