珊瑚猴头菌粗多糖对DSS诱导的小鼠慢性肠炎的预防作用

The prevention effects of Hericium coralloides polysaccharides (HCP) on chronic colitis and the impact of HCP on gut microbial communities in mice were investigated. Sixty mices were randomly divided into six groups: blank control (CK), model control (MC), positive control (PC), low-dose HCP (L-HCP), medium-dose HCP (M-HCP), and high-dose HCP (H-HCP). Biochemical indicators of serum, colonic tissue and histopathological sections were compared among these groups, and 16S rRNA high-throughput sequencing was used to analyze the relative abundance and the diversity of the intestinal microflora at the phyla and class levels. Results showed that HCP significantly reduced the levels of serum interleukin-1β (IL-1β), serum interleukin-2 (IL-2), serum interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) in the serum, and decreased the levels of the Toll-like receptor 4 (TLR4), cysteine protease 1 (Casp-1), apoptosis-associated speck-like protein (ASC) and phosphorylated p65 protein (pp65) in colonic tissue. The 16S rRNA sequencing showed that HCP significantly increased the α-diversity indices Chao1, Shannon, and observed species, reduced the Simpson index, and regulated the abundance of Bacteroides and Firmicutes, thereby reducing colonic tissue damage. In conclusion, HCP can prevent DSS-induced chronic colitis and possesses potential utilization value in the field of functional food.