BEEM : Biomass enhancement under eskimo1 mutation

To allow identification of causal snp involved in eskimo1 dwarfism suppression (beem) after an ems treatment of esk1 mutant, plants with larger rosettes than those of esk1 mutant were selected and back-crossed esk1 mutant. In case of recessive mutation, the F1 progeny presented esk1 phenotype and werer selfpollinated. In case of dominant mutation, the F1 progeny were crossed with esk1 and the next generation produced 100% beem phenotype. All beem lines were submitted to 5 cycles of backcross(BC) with esk1 mutant and F2 segregation observation. In F2BC5, bulk of 10 main inflorescences of each phenotype (beem and esk1) were collected by beem line. DNA of these bulks were extracted and whole genomes were sequenced.