Effects of triiodothyronine and/or Irisin on human subcutaneous adipocytes: a global transcriptome analysis

In order to investigate, at the mRNA level, the signaling pathways through which triiodothyronine (T3) and irisin (I) affects Human subcutaneous adipocytes function, human Cultured Human subcutaneous preadipocytes were differentiated into mature adipocytes, which were cultured with T3 and/or Irisin, the control group was without treatment. RNA-Seq was performed using Illumina platform, and differential gene expression was assessed with DESeq2. Among differentially expressed genes, enrichment analysis was performed for biological processes against the Gene Ontology Consortium database, using both ClusterProfiler R package and STRING. Grant 2016/03242-3, São Paulo Research Foundation (FAPESP). Grant 409438/2016, National Council for Scientific and Technological Development (CNPq). Overall design: The study consists of four groups with three biological replicates each. Human subcutaneous adipocytes cells were cultured for 24h with T3 (10nM) or Irisin (20nM) and T3 plus Irisin (T3+Irisin), for the without treatment control group.