Striking variation of pollinator attracting scent within a highly specialized pollination system

Although attractive scents play a crucial role in reproduction in insect-pollinated plants, the degree of variation of this signal within and among populations remains understudied. Depending on the specifics of the reproductive system of the plant under scrutiny, it is possible to formulate predictions regarding this variation. In plants with separate sexes (dioecious species) and with highly specific pollination, one would predict (i) males to emit more scent than females, owing to sexual selection, (ii) scent bouquets to have a strictly similar composition between sexes, to guarantee efficient pollen transfer and (iii) variation of scent bouquet among populations that should mirror neutral genetic divergence. These hypotheses were tested in the European fan palm, Chamaerops humilis, by collecting scent in eight populations from three regions, Spain, Sardinia and Sicily, quantifying densities of pollinators - Derelomus sp. and Meligethinus pallidulus - and genotyping the plants on a s..., Three types of data, described below, were collected for our study: microsatellite genotypes, pollinator observations, and odors emitted by the leaves of the dwarf palm Chamaerops humilis. Data was collected from two Spanish (SPA1 and SPA2), three Sicilian (SIC1, 2 & 3), and three Sardinian natural populations (SAR1, 2 & 3) of Chamaerops humilis. Data was obtained as follows:
MICROSATELLITE DATA : 377 plants from the eight populations were genotyped using thirteen neutral microsatellite markers (see 'Genotype' tab, missing allles are coded as \"000\"). Six of these markers were developed by Guarino et al. (Molecular Ecology Resources Primer Development, 2013, marker names : GRD72UV01A0I0S, GRD72UV01BCJA2, GRD72UV01BR6MX, GRD72UV01AW9C6, cons470, GRD72UV01BS052), and seven were developed for the needs of this study and have not yet been published (Ch09, Ch14, Ch15, Ch18, Ch21, Ch30, Ch52). The primers for the newly developped markers are available in the 'Primers' tab. Three single..., , # Striking variation of pollinator attracting scent within a highly specialized pollination system

[https://doi.org/10.5061/dryad.xksn02vs0](https://doi.org/10.5061/dryad.xksn02vs0)

## Description of the data and file structure

Three types of data, described below, were collected for our study: microsatellite genotypes, pollinator observations, and odors emitted by the leaves of the dwarf palm *Chamaerops humilis*. Data was collected from two Spanish populations (SPA1 and SPA2), three Sicilian populations (SIC1, 2 & 3), and three Sardinian populations (SAR1, 2 & 3).

### Files and variables

#### File: Chumilis\_Data.xlsx

**\"Primers\" tab : Microsatellite primers designed for the study.**

Variables :

* Marker : Name of microsatellite marker
* Dye : Fluorescent dye used for electrophoresis
* Repeat motif : Type of repeat motif
* Forward sequence
* Reverse sequence
* Size range (bp) : Allele size in base pairs

**\"Genotypes\" tab : Genotypes for the 377 *Chamaerops humilis* in...