T cell maturation, selection, and function differentially depend on the chaperonin CCT

T cell homeostasis rely on de-novo transcription of proteins to ensure antigen-specific immunity. Central in this process and to ensure efficient and correct protein folding and turn-over is the Eukaryotic Type II chaperons, which primary targets are actin and tubulin. So far its role in T cell development and function remain unclear. Using a conditional deletion of a subunit within the type II chaperon complex we show this complex to be indispensable for correct maturation of thymocytes, with a reduced thymic output of mature effector and regulatory T cells. Accordingly, we found the formation of nuclear actin filaments to be reduced resulting in an array of functional effects with the most prominent being the failure to commit to the Th2 cell linage with a further insufficiency in recruiting cellular and humoral effector mechanisms to command an effective anti-parasitic response. Hence, our results highlight the importance of the Eukaryotic Type II chaperons in shaping the fate and function of CD4 T cells. Overall design: RNA-seq in wild-type and Cct8 KO in lymphocytes