Profiling in vivo Bone Lesion (IVBL) and Orthotopic tumors by Next Generation Sequencing

Based on RNA-seq, we performed transcriptomic profiling to examine the differences between Orthotopic and IVBL (in vivo bone lesion). We found Calcium signalling is upregulated in IVBL and correlated to the expression of gap junctions. Overall design: Orthotopic tumors and Bone lesions, all developed by MCF-7, are subject to NGS and then analyzed.