Identification of N6-methyladenosine functions of YTHDF3 using MeRIP-seq analysis combined with transcription sequencing

We performed methylated RNA immunoprecipitation followed by sequencing (MeRIP–seq) combined with transcription sequencing to clarify the mechanism underlined YTHDF3 in CRC Overall design: More than 50 ug of total RNA is sufficient for followed RNA fragmentation and immunoprecipitation according to the manuscript of the Magna MeRIP™ m6A Kit (Merck, Darmstadt, Germany).