Transcriptome analysis of Klebsiella quasipneumoniae colonized in the mouse intestine

Klebsiella pneumoniae is a Gram-negative, non-motile, facultative anaerobic, rod-shaped bacterium. Klebsiella is known as the causative bacterium of pneumonia and urinary tract infections. In recent years, it has been reported that the colonization of Klebsiella in the intestinal tract causes excessive activation of TH1 cells and may be involved in the development of inflammatory bowel disease. Therefore, we have performed a transcriptomic in vivo study with Klebsiella quasipneumoniae subsp. similipneumoniae strain ATCC 700603 in the intestine of living mice to identify novel latent and adaptive fitness determinants within K. quasipneumoniae. From the 5171 gene present in K. quasipneumoniae strain 700603, we identified several genes that are significantly differentially expressed in the intestinal tract of living mice compared to exponential growth in LB broth.