Data from: A phylogeny and evolutionary natural history of Mesoamerican toads (Anura: Bufonidae: Incilius) based on morphology, life history, and molecular data

We combine mitochondrial and nuclear DNA sequence data with non-molecular
(morphological and natural history) data to conduct phylogenetic analyses
and generate an evolutionary hypothesis for the relationships among nearly
every species of Mesoamerican bufonid in the genus Incilius. We collected
a total of 5,898 aligned base-pairs (bp) of sequence data from
mitochondrial (mtDNA: 12S–16S, cyt b, ND2–CO1, including tRNAsTRP–TYR and
the origin of light strand replication; total 4,317 bp) and nuclear (CXCR4
and RAG1; total 1,581 bp) loci from 52 individuals representing 37
species. For the non-molecular data, we collected 44 characters from 29
species. We also include Crepidophryne, a genus that has not previously
been included in molecular analyses. We present results of parsimony and
Bayesian analyses for these data separately and combined. Relationships
based on the non-molecular data were poorly supported and did not resolve
a monophyletic Incilius (Rhinella marina was nested within). Our molecular
data provide significant support to most of the relationships. Our
combined analyses demonstrate that inclusion of a considerably smaller
dataset (44 vs. 5,898 characters) of non-molecular characters can provide
significant support where the molecular relationships were lacking
support. Our combined results indicate that Crepidophryne is nested within
Incilius; therefore, we place the former in the synonymy of the latter
taxon. Our study provides the most comprehensive evolutionary framework
for Mesoamerican bufonids (Incilius), which we use as a starting point to
invoke discussion on the evolution of their unique natural history traits.