Data associated with "Correlative Raman and immunofluorescence imaging reveals different protein abundance between stress granules induced by oxidative damage"

Figures Figure S1: Representative IF images of cells treated under increasing NaAsO2 or H2O2 concentrations at the indicated durations Figure S2: Representative IF images of cells under each treatment labeled with anti-G3BP1 Figure 1A: Relative reactive oxygen species generated by each stress treatment measured by DCFH-DA assay Figure 1B and S3: Western blot of Nrf2 protein levels in stressed cells and corresponding Ponceau-S stain Figure 2A, 2B, and 2C: Representative IF images of cells under each treatment labeled with anti-G3BP1, anti-eIF4G, and anti-TDP-43 antibodies taken at confocal and super resolution Figure 2D: Number of cells per 100 counted which have a SG-phenotype determined by IF staining Figure 2E: Average number of SGs produced per SG-positive cell determined by IF staining Figure 3A: Excitation and emissions spectra of Alexa405 and Alexa647 dyes taken on a fluorometer Figure 3B: Representative multi-layer IF image of cells treated with sodium arsenite for 1 h and labeled with anti-G3BP1 and anti- Figure 3C: Averaged Raman spectrum of a cell focused on the C–H stretching region after background subtraction Figure 3D–G: Raw RSI map data used to generate maps of lipid, protein, and first moment analysis Figure 3H: Averaged Raman spectra of outlined puncta and cytosol identified by first moment analyses in Fig. 3G Figure S4: Integrated area under CH3 stretch of Raman spectrum taken at indicated Z-position through a single point in a cell Figure 4: Raw RSI map data used to generate first moment analyses and correlative widefield fluorescence images of anti-G3BP1 Figure 5: Averaged Raman spectra of outlined puncta and adjacent cytosol in Fig. 4 Figure 6, S5, S6, and S7: Correlative, multi-layer IF images and raw RSI map data used to generate z-stacks