RNA-seq analysis for transcriptome changes during Pep1-induced salt tolerance in Arabidopsis

This analysis aims to have an overview for the effects of Pep1 application on transcriptome changes following subsequent exposure to salt stress in Arabidopsis. Five-day-old wile-type (Col-0) and pepr1-1 pepr2-3 seedlings grown on agar plates containing 1/2 x MS and 25 mM sucrose were treated with or without 0.1 µM Pep1 in liquid culture for 3 days, and then to 150 mM NaCl for the indicated times (3 h and 24 h). Total RNA was prepared and subjected to RNA-seq analysis by HiSeq2500 with Single-read 50 bases. pepr1-1 pepr2-3 was shown to be insensitive to Pep1 and used as the negative control for normalization of the data.