Knockdown of hCdc14B leads to increase of the mitotic index, multinucleation and apoptosis.

HeLa Kyoto cells transfected with hCdc14B-specific siRNA-3 (siCdc14B-3) or non-targeting siRNAs (siCtrl) were synchronized as shown in Fig. 3A and B. Mitotic cells with condensed chromatin were counted 8–16 h after release from G1/S, multinucleated cells were counted 8 h after release, and apoptotic cells were visualized by TUNEL staining 24 h after release. Numbers represent the mean levels (±SD) from three independent experiments comprising counts of 400 cells each.