MC_JAK1_validation_amplicon_seq_exp1_2_010321
收藏NIAID Data Ecosystem2026-03-13 收录
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资源简介：
amplicon sequencing of gene edited loci. Supplied are 144 PCR1 reactions (50 ul), of 300 bp -350 bp in length requiring gel QC, SPRI bead clean-up, quantification, PCR2, SPRI bead clean-up, equimolar pooling and PE sequencing. standard PCR1 adapters used: For. ACACTCTTTCCCTACACGACGCTCTTCCGATCT Rev. TCGGCATTCCTGCTGAACCGCTCTTCCGATCT Can proceed with PCR2 indexing using the standard protocols (e.g. for CRISPR PCRs), noting the larger amplicon size.
创建时间：
2022-03-22
