The F-box protein Fbx23 regulates the development of Penicillium oxalicum fungi and the expression of cellulolytic genes

The goals of this study are to compare different gene expressions for Penicillium oxalicum wild type strain (WT), Fbx23 deletion strain (Δfbx23), and Fbx23 ecover strain (Refbx23) under conditions of 2% glucose or 1% bran + 1% microcrystalline cellulose as carbon source. The results of correlation analysis between each sample showed that the gene expression level of Δfbx23 was significantly different from that of the WT strain. The deletion of Fbx23 significantly down-regulates the expression levels of sporulation process genes. And the absence of Fbx23 can broadly up-regulate the expression level of cellulase-encoding genes, indicating that Fbx23 can cause a decrease in cellulase synthesis by affecting the expression of cellulase-encoding genes. Examination of differential gene expressions by digital gene expression tag profiling in Penicillium oxalicum wild type strain, Fbx23 deletion mutant strains and Fbx23 recover strain in 18h in modifeid Mendal’s medium with 2% glucose or 1% bran + 1% microcrystalline cellulose as carbon sources. Three replicates were included for each samples.