Balanced act of a leading strand DNA polymerase specific domain and its exonuclease domain promotes genome-wide replication fork symmetry

Replicon-seq was used to analyze DNA replication in polymerase epsilon mutants in budding yeast. Replicon seq utilizes Micrococcal Nuclease (MNase) fused to the replicative helicase MCM in vivo. Cells are grown in the presence of BrdU and site-specific cleavage is induced at sites of DNA replication in S phase with the addition of calcium. The resulting DNA molecules are extracted and sequenced using Nanopore. Molecules containing BrdU are selected and analyzed. The data in this record contain biological replicates for two mutants of DNA polymerase epsilon: REL and REL-Exo-.