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N-cadherin dynamically regulates pediatric glioma cell migration in complex environments

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DataCite Commons2025-06-01 更新2025-04-10 收录
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https://datadryad.org/dataset/doi:10.5061/dryad.4tmpg4fj7
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## N-cadherin dynamically regulates pediatric glioma cell migration in complex environments Table S2. Control vs. N-cadherin shRNAs. We compared RNA transcriptomes from bulk cell populations, control or N-cadherin-depleted pediatric high-grade glioma cells. N-cad depletion decreased RNA expression but unaffected the expression of other cadherins and integrins except for CDH3. Differential gene expression analysis was performed with the DEseq2 for paired sample R package. Table S3. Leader vs. follower cells. We compared RNA transcriptomes from migrating glioma leader and follower cells that we isolated by photoconversion and flow cytometry. 44 gene transcripts increased and 36 decreased in leader relative to followers out of 19,729 genes that were quantified (log2 fold-change >0.5, FDR <0.05). YAP-response genes and wound-healing genes were higher in leader than follower cells. Differential gene expression analysis was performed with the DEseq2 for paired sample R package. NA represents no count. ### Description of the data and file structure The RNA sequencing data comprises two sheets in one Excel file. Table S2 is Control and N-cad shRNA samples, and Table S3 is Leader and follower cells. The first sheet presents the differential gene expression analysis results for paired samples from four biological replicates. Adjusted p-values are set to NA due to zero count or extreme count outliers. In the second sheet, normalized gene counts for each sample are provided. The first row in each sheet contains the sample names, with corresponding paired sample numbers indicated. The first column represents gene symbols.
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Dryad
创建时间:
2024-01-15
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