N-cadherin dynamically regulates pediatric glioma cell migration in complex environments
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## N-cadherin dynamically regulates pediatric glioma cell migration in
complex environments Table S2. Control vs. N-cadherin shRNAs. We compared
RNA transcriptomes from bulk cell populations, control or
N-cadherin-depleted pediatric high-grade glioma cells. N-cad depletion
decreased RNA expression but unaffected the expression of other cadherins
and integrins except for CDH3. Differential gene expression analysis was
performed with the DEseq2 for paired sample R package. Table S3. Leader
vs. follower cells. We compared RNA transcriptomes from migrating glioma
leader and follower cells that we isolated by photoconversion and flow
cytometry. 44 gene transcripts increased and 36 decreased in leader
relative to followers out of 19,729 genes that were quantified (log2
fold-change >0.5, FDR <0.05). YAP-response genes and
wound-healing genes were higher in leader than follower cells.
Differential gene expression analysis was performed with the DEseq2 for
paired sample R package. NA represents no count. ### Description of the
data and file structure The RNA sequencing data comprises two sheets in
one Excel file. Table S2 is Control and N-cad shRNA samples, and Table S3
is Leader and follower cells. The first sheet presents the differential
gene expression analysis results for paired samples from four biological
replicates. Adjusted p-values are set to NA due to zero count or extreme
count outliers. In the second sheet, normalized gene counts for each
sample are provided. The first row in each sheet contains the sample
names, with corresponding paired sample numbers indicated. The first
column represents gene symbols.
提供机构:
Dryad
创建时间:
2024-01-15



