AMPK-dependent gene expression profiles in response to small molecule AMPK activators

AMP-activated protein kinase (AMPK) is a major regulator of cellular energy homeostasis that coordinates metabolic pathways in order to balance nutrient supply with energy demand. AMPK elicits acute and diverse metabolic effects by directly phosphorylating various targets. AMPK activation also promotes metabolic reprogramming in longer term via effects on gene expression. The aim of this study is to elucidate molecular mechanism(s) by which AMPK activation modulates metabolic adaptation through its impact on gene regulation. The mouse embryonic fibroblasts (MEFs) dataset comprises a) two genetic backgrounds (control and AMPK α1-/-/α2-/-), b) three treatments (vehicle, 991, AICAR), and c) four technical replicates per condition. One sample was excluded as outlier (control, 991-treated, technical replicate 1) for a total of 23 samples. The primary hepatocytes expression profiling was generated using a) two genetic backgrounds (control and AMPK α1-/-/α2-/-), b) three treatments (vehicle, 991, AICAR), c) five technical replicates, and d) two biological replicates. One sample was excluded as outlier (AMPK α1-/-/α2-/- vehicle-stimulated, technical replicate 4, biological replicate 1) giving finally 59 samples.