Isopropyl 3-(3, 4-dihydroxyphenyl)-2-hydroxypropanoate protects lipopolysaccharide-induced acute lung injury in mice by attenuating pyroptosis- Supplemental Material

This dataset is supplemented to the manuscript entitled 'Mei-Ling Zhang1, Meng Wang1, Jian Chen1, Yan-Jie Liu1, Ya-Jie Yu, Li-Min Liu, Xiao-Hui Zheng, Ying-Chou Xiao, Jun-Ming Zhang, Meng-Xue Zhu, Xian Yue, Ye Zhao*, Wen Niu* and Zhi-Chao Li*, Isopropyl 3-(3, 4-dihydroxyphenyl)-2-hydroxypropanoate protects lipopolysaccharide-induced acute lung injury in mice by attenuating pyroptosis'.
Appendix 1. Immunofluorescence staining analysis of Gsdmd expression (red stain) and the LPS-stimulated macrophages marker CD68 (green stain). The nuclei (blue stain) were counter-stained using DAPI staining solution.
Appendix 2. Assays for apoptosis of THP-1 macrophages. (a) Control and (b) 180 μg/ml of IDHP administration.
Appendix 3. Cell viability was measured by CCK8 assay.
Appendix 4. Flow cytometry analyzed ROS production in THP-1 macrophages stimulated by LPS with or without IDHP pretreatment: (a) Control, (b) IDHP (180 μg/ml), (c) LPS (1 μg/ml), (d) IDHP (180 μg/ml) + LPS (1 μg/ml).