Transcriptional Heterogeneity of Progression in MYOD1-mutant spindle cell rhabdomyosarcoma

We perform single nuclei transcriptomics (snRNA-seq) analysis of patient-derived tumor samples to define the dynamic myogenic cell states and intratumoral transcriptional heterogeneity of MYOD1-mutant spindle cell/sclerosing rhabdomyosarcoma. Overall design: Primary and recurrent/metastatic, snap-frozen patient-derived samples (n = 6) of MYOD1-mutant spRMS were profiled by snRNA-seq using the 10X Genomics platform.