Physiological state matching in a pair bonded poison frog

# Title of Project: Physiological state matching in a pair bonded poison frog Access preprint version at bioRxiv at: [https://doi.org/10.1101/2022.09.25.509360. ](https://doi.org/10.1101/2022.09.25.509360.) The following raw datasets have been uploaded: 1-Empathy.txt, 2-CORTcorr.txt and 3-bhs.txt ### Descriptions of datasets: ***1- ‘Empathy’***: the concentration of corticosterone of every individual and information related to their experimental condition (i.e. partner/non-partner, stress/non-stress, baseline/treatment/housing tank). * *Date_Plate*: The date on which the ELISA hormonal plate was run (dd.mm.yy) * *Plate:* The number of the ELISA plate that the sample was on * *Date*: The date of hormone data collection (mm.dd.yy) * *Date2:* The date of hormone data collection (dd.mm.yy) * *Sex:* the sex of focal individual * *ID*: the ID of the focal individual * *CoupleID*: the ID of the focal individual's partner/non-partner female * *Condition*: the experimental condition of data collected * *Condition2:* for the experimental animals, further detail on experimental condition- either pre-treatment (Baseline) or one of the post-treatment conditions (non-stress or stress) * *Familiarity:* whether the focal individual was assayed with their partner or a non-partner * *Trial:* Whether the condition was pre-treatment (Baseline) or post-treatment (Treatment) * *CORT_conc:* the corticosterone concentration of the focal individual ***2- ‘CORTcorr’***: A ‘widened’ version of the ‘Empathy’ data set, where corticosterone concentrations are paired based on the experimental dyads. Because some of the hormone samples did not meet the quality controls (i.e. intra-assay CV > 20%) we kept only pairs that had pre and post-experimental measurements in all four experimental conditions (partner + non-stress, partner + stress, non-partner + non-stress, non-partner + stress). * *MDate_Plate:* The date that the ELISA plate was run * MPlate: the number of the plate on which the ELISA was run * *MDate*: The date of hormone data collection (mm.dd.yy) * *MDate2:* The date of hormone data collection (dd.mm.yy) * *CoupleID*: the ID of the focal individual's partner/non-partner female * *MSex:* the sex of focal individual-male * *MID*: the ID of the focal individual-male * *MCondition:* the post-treatment experimental condition (stress or non_stress) * *MFamiliarity:* the familiarity condition (partner or non_partner) * *MTrial*: Whether the sample was collected from pre-treatment (Baseline) or post-treatment (Treatment) * *MCORT_conc:* the corticosterone concentration of the focal individual-male * *FSex*: the sex of the partner-female * *FID:* the ID of the partner-female * *FCORT_conc*: the corticosterone concentration of the focal individual-female ***3- ‘bhs’***, Values of 9 scored individual and joint (male-female dyad) behaviors of every individual frog in every experimental condition. * *State*: whether data was collected pre (Pre) or post (Post) treatment * *Familiarity*: whether animal was assayed with partner (Partner) or non-partner (Non-partner) * *Condition:* Experimental condition of animal (Baseline, Non-Stress, or Stress) * *Sex:* sex of focal individual * *Subject*: individual identification (ID) * *Activity_level*: Total number of times animal is in motion, either stationary or through space * *CordFreeezingB*: Both animals are freezing at same time- total number of bouts * *CordFreezingD*: Both animals are freezing at same time- total duration (seconds) * *FreezingB*: Total number of times animal is motionless for at least 1 min. and completely visible * *FreezingD:* Total duration of time animal is motionless for at least 1 min. and completely visible (seconds) * *JointRefuge:* Both animals are refuging together in moss or cup * *PartnerApproachB*: Total number of times animal moves towards other conspecific * *PartnerApproachD:* Total duration of time animal moves towards other conspecific (seconds) * *PartnerGazeB:* Total number of times animal is facing towards other conspecific * *RefugeUse:* Total number of times animal is attempting to be concealed within moss or in cup * *AV_speed_per_sec_cm*: Average speed animal has moved through space (cm per second) * *tot_distance_moved_cm:* Total distance animal has moved (cm) * *Total_area_used_cm2:* Total area of space that the animal used (cm2) * *AV_M.F_proximity_cm*: The average distance between animals (cm) * *Couple:* numeric ID assigned to each dyad *Table of descriptions of coded behaviors* | Behavior | Description | Scoring.software | | :--------------------------- | :------------------------------------------------------------------------------------- | :--------------- | | **Individual** | | | | Gaze - bouts | Total number of times animal is facing towards other conspecific | BORIS | | Gaze - duration | Total duration animal is facing towards other conspecific | BORIS | | Approach - bouts | Total number of times animal moves towards other conspecific | BORIS | | Approach - duration | Total duration of times animal moves towards other conspecific | BORIS | | Freeze - bouts | Total number of times animal is motionless for at least 1 min. and completely visible | BORIS | | Freeze - duration | Total duration of time animal is motionless for at least 1 min. and completely visible | BORIS | | Refuge use - duration | Animal is attempting to be concealed within moss or in cup | BORIS | | Activity - bouts | Total number of times animal is in motion, either stationary or through space | BORIS | | Activity - speed | Average speed animal has moved through space | Annolid | | Activity - distance | Total distance animal has moved | Annolid | | Space use | Total area of space that the animal used (cm2) | Annolid | | **Male-female dyad** | | | | Male-female proximity | The average distance between animals | Annolid | | Male-female joint refuge use | Both animals are refuging together in moss or cup | BORIS | | Coordinated freezing | Both animals are freezing at same time | BORIS | For further accompanying supplemental materials, including code, tables, and figures, please see: [https://rpubs.com/camilo-rl/Physiological_state_matching_in_a_pair_bonded_poison_frog-Data_analysis](https://rpubs.com/camilo-rl/Physiological_state_matching_in_a_pair_bonded_poison_frog-Data_analysis).