Polarization of yeast cells under different pheromone induction regimens

The yeast Saccharomyces cerevisiae undergoes polarized growth in two different contexts: budding and pheromone-induced mating. A key difference between the two processes is that mating polarization occurs in response to an external stimulus, which can be easily manipulated under laboratory conditions.  We exploited this property to test the hypothesis that the underlying polarity circuit is bistable.  To make this test we used microfluidics to precisely control the temporal profile of mating pheromone and live-cell imaging to monitor polarization in single living cells. Files posted here contain single cell polarity time course data under different pheromone regimens. These data clearly demonstrate that polarization as a function of pheromone concentration depends on the history of the system, a behavior that is a hallmark of bistability. The data also re­veal that polarity is rapidly lost following a sudden loss of phero­mone., The accompanying \"Data_Information\" document explains how polarization was visualized in living cells, the metric used to quantify polarization and a Table summarizing the pheromone regimens under which the data were collected., , # Polarization of yeast cells under different pheromone induction regimens

[https://doi.org/10.5061/dryad.4f4qrfjn0](https://doi.org/10.5061/dryad.4f4qrfjn0)

Data sets posted here were used to support the experimental observations presented in \"Multiscale modeling of bistability in the yeast polarity circuit\" (Hladyshau, Guan et al., 2024). A separate file, \"Data_Information.docx\", briefly describes the data and provides details for the computational methods used to analyze the data. Full details for the experimental methods can be found in (Vered 2018) \"Memory and bistability in the pheromone response pathway.\" Ph.D. Dissertation, University of North Carolina at Chapel Hill. It should be noted that the data in the posted files are from analyses and some trials made independently from those for figures appearing in the Vered thesis dissertation (Vered 2018).

## Description of the data and file structure

Data were collected for polarity establishment in yeast responding to mating ph...