Bacterial 16S rRNA community in manganese reducing slurry incubation experiment with sediment of Potter Cove

An incubation experiment was performed, investigating manganese reduction and the associated microbial community in Antarctic, glacial influenced sediments. Slurry was prepared in a 1:5 ratio of sediment and sulfate-free artificial sea water. The used sediment originated from core STA01.02, 0-25 cm, at station 01 in Potter Cove, King George Island/Isla 25 de Mayo, West Antarctic Peninsula, retrieved in Austral summer 2019. The incubation experiment was performed anoxically for two treatments: birnessite (manganese oxide) + acetate and acetate-only, in biological triplicates, incubated at 2 degrees C in the dark. DNA and RNA were co-extracted and the present and active bacterial community was identified by 16S rRNA gene amplicon sequencing on RNA (cDNA) and DNA level. Project was funded by DFG SPP 1158