Effect of NS1 alanine substitutions on DENV reporter virus replication.

The replication level of a given mutant relative to the wild-type (WT) is displayed. Replication was determined by measuring luciferase activity 48 h post-transfection and normalizing to the 4 h value that reflects transfection efficiency. Values represent mean ± standard deviation of three independent experiments. NS1 mutants with severe defects in viral RNA replication are underlined, while asterisks (*) indicate mutants with selective defects in virus assembly or release.Effect of NS1 alanine substitutions on DENV reporter virus replication.