Dataset periodontitis y2021m02

Description and susceptibility
of periodontopathic bacteria with severe chronic periodontitis among a group of
Peruvian patients: A pilot study

ABSTRACT

Background: Severe periodontal disease is
highly prevalent worldwide, affecting 20 % of the population between the ages
of 35 and 44 years old. The etiological epidemiology in Peru is scarce, even
though some studies describe a prevalence of 48.5 % of periodontal disease in
the general population. Periodontitis is one of
the most prevalent oral diseases associated with site-specific changes in the
oral microbiota and it has been associated to socioeconomic state. This study aimed to determine
the etiology and resistance profile of bacteria identified in a group of
patients with chronic severe periodontitis.

Methods: Six subgingival plaque
samples were collected from eight patients with chronic severe periodontitis.
Bacterial identification was carried out by an initial culture, PCR
amplification and subsequently DNA sequencing. We evaluated the antibiotic
susceptibility by disk diffusion method.

Results: Variable
diversity in oral microbiota was identified in each one the eight patients. The
bacterial genus most frequently found was Streptococcus
spp. (15/48, 31.3 %) followed by Rothia
spp. (11/48, 22.9 %), Actinomyces
spp. (9/48, 18.8 %), and Eikenella
spp. (4/48, 8.3 %). The most common species found was Rothia dentocariosa (8/48, 16.7 %). The antimicrobial
susceptibility assay varied according to the species tested, however, among all
the isolates evaluated Actinomyces
naeslundii was resistant to penicillin and tetracycline; Eikenella corrodens resistant to
dicloxacillin; and Rothia dentocariosa
was resistant to amoxicillin + clavulanic acid and metronidazole but also susceptible
to trimethoprim-sulfamethoxazole.

Conclusions:
Bacterial species of importance medical were detected in patients with chronic
severe periodontitis, especially Rothia
dentocariosa, most prevalent in this study. Specific antimicrobial therapy
is required to improve the treatment outcomes of patients with periodontal
disease and to avoid the development of antibiotic resistance.