Pseudomonas putida KT2440 Genome sequencing

In this study, we overcome the limitations typically associated with traditional biosensors by engineering Pseudomonas putida for whole-cell sensing of chemicals. Our approach overcomes previous limitations by integrating synthetic auxotrophies within central carbon metabolism, which can be complemented by a series of target analytes, with fluorescent reporters in growth-coupled setups. This platform enables the detection of a wide array of structurally diverse chemicals under various conditions, including co-cultures of producer and sensor strains. This system provides a rapid, sensitive, and readily adaptable tool for monitoring microbial factory performance and for environmental analyses.The submitted sample is the sensor strain evolved for the detection of terephthalic acid (TPA), the GclR regulator deleted, and with the MHETase integrated.