RNA-seq analysis of meristem cells in cultivated strawberry.

The goal of this project is to determine the differentially expressed genes during the transition from the vegetative to reproductive meristem cells in a cultivated strawberry 'Tochiotome'. To achieve this goal, plants were grown under floral inductive condition (short day and low temperature) or non-floral inductive condition (long day and high temperature) and meristem cells were isolated using a laser microdissection (LM) technique. Then, total RNA was extracted from the LM-isolated tissues and RNA-seq libraries were prepared with the SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Clontech Laboratories) and the Low Input Library Prep Kit v2 (Clontech Laboratories). The libraries were sequenced on HiSeq2000 (illumina) with paired-end 100 bp.