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TAT.ARC protein transduction or RNA interference with ARC impact on survival of primary cortical neurons subjected to 150 min oxygen-glucose deprivation (OGD): Lactate dehydrogenase activity was measured as a marker of cell membrane integrity loss and neuronal cell death 24 h afterwards.

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https://figshare.com/articles/dataset/TAT_ARC_protein_transduction_or_RNA_interference_with_ARC_impact_on_survival_of_primary_cortical_neurons_subjected_to_150_min_oxygen-glucose_deprivation_OGD_Lactate_dehydrogenase_activity_was_measured_as_a_marker_of_cell_membrane_integrity_loss_and_neuron/3406282/1
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Primary neuronal cultures were transduced with lentiviral particles for knockdown of apoptosis repressor with CARD domain (ARC) or non-targeting control or treated with TAT proteins for ARC or beta Galaktosidase before 150 min of oxygen glucose deprivation (OGD). Cell death was monitored by the release of lactate dehydrogenase (LDH) in the supernatant medium.
提供机构:
figshare
创建时间:
2016-08-04
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