Sheep rump fat LC-MSMS

In this study, we established a persistent starvation model to imitate the two extreme states of rump fat deposition and mobilization. Simultaneously, expression patterns of lipid metabolism-related genes in rump fat, selected using iTRAQ, were assessed before and after the starvation test. The rump-fat sectioning data showed a positive correlation of cell size with fat deposition ability. iTRAQ proteomic analysis facilitated the detection of ~1610 proteins in Altay rump fat, among which 112 displayed significant differences in expression between the two extreme states. On this basis, we observed increased secretion of LEP and ADIPOQ and simultaneous activation of key fat mobilization and fatty acid oxidation signal pathways, such as AMPK and PPAR, under persistent starvation and extreme cold conditions.