Transcriptomic analysis of murine lung tumor cells upon genetic KrasG12V ablation

To identify the mechanisms driving resistance upon KrasG12V ablation, we established lung cancer cell lines that carried loxP sequences flanking the exon 1 of Kras containing the G12V mutation (Kras +/loxG12Vlox), and lacked Trp53 alleles (Trp53 -/-). Tumor cells were infected with Adeno-Cre particles to excise the floxed sequences and individual cells that survived were expanded for further analysis. Gene expression profiling analysis of parental lung tumor cells Kras (+/loxG12Vlox);Trp53(-/-) and resistant lung tumor cells Kras (+/-);Trp53(-/-)