Sensitivity testing of each cPCR and qPCR marker across a dilution series of genomic DNA. The proportion of detections out of four replicates is noted for each marker at each dilution level. Estimated
A “+" sign indicates that a PCR product was detected. A “−" sign indicates that no PCR product was detected, suggesting that a truncated BAC construct was integrated in these lines.
Assay validation is an essential component of disease surveillance testing, but can be problematic in settings where access to positive control material is limited and a safety risk for handlers. Here
