Regional specialization and fate specification of mesenchymal stromal cells in skeletal development [bulk RNA-seq]

To gain insight into the molecular signatures and heterogenity of bone mesenchymal stromal cells population between metaphysis (mpMSC) and diaphysis (dpMSC). mpMSCs and dpMSCs were sorted from three weeks old Hey1-GFP metaphysis and Pdgfra-EGFP BM using a flow cytometer for bulk-RNA sequencing analysis. Bulk-RNA sequencing -mpMSC and dpMSC bulk-RNA seq. libraries prepared by using SMARTer Ultra Low input RNA kit-V3 and NexteraXT DNA library prep kit.