Phosphoproteome analysis of signaling by ErbB2 in ovarian cancer cell line(1st approach)

The gene for receptor tyrosine kinase ErbB2 is amplified in breast and ovarian tumours. The linear pathway by which signals through ErbB2 are transduced is well known. However, second generation questions that address spatial aspects of signaling remain. To address this, we have undertaken a mass spectrometry approach to identify phosphoproteins specific for ErbB2 using the inhibitors Lapatinib and CP724714 using affinity chromatography and nano-LC ESI MS/MS