Paeonia lactiflora extract increase the muscle function via downregulation of high mobility group box 1 and NF-kB signaling in mdx mice, an animal model of Duchenne muscular dystrophy

Mouse gastrocnemius muscle tissue was collected from three groups: wildtype (N1, N2, N3), mdx (C1, C2, C3), and mdx+PL (RP1, RP2, RP3). Proteins were digested to peptide mixtures using FASP and analyzed using LC-MS/MS. The DIA files for individual sample were processed using mouse reference spectral library MouseRefSWATH(PASS01569). The results of protein quantification were further processed and analyzed using R (ver. 3.6.0).