Supporting Data for 'Kinase Library Screen identified VRK3 as a Novel DNA Damage Factor'

Data from the CRISPR library screen enabled us to isolate the target protein in this research. Using live-cell imaging, we were able to validate the involvement of the hits from the CRISPR library screening. Laser microirradiation experiments coupled with live-cell imaging to study the temporal accumulation kinetics of targets that participate in DNA damage repair. We also utilized several DNA damage markers in immunofluorescence staining upon DNA damage induction. The interactions between the target protein and DNA damage factors were also explored using co-immunoprecipitation and pull-down assays, as shown in the western blot data.